by plotting the absorbance of standard dye solutions at lambdamax against the co

Question

by plotting the absorbance of standard dye solutions at lambdamax against the corresponding dye concentrations, the calibration curve should be a linear line, and the slope of the calibration curve is epsilon l. In accordance with Beer?s Law, the slope of the linear trend line on a plot of absorbance versus concentration is equal to . the product of the molar extinction coefficient and the path length the molar extinction coefficient the transmittance the light path length the equilibrium constant Concept: Beer?s law is the linear relationship between absorbance and concentration of an analyte. It is written as: A = epsilon/C Where: A = Absorbance of the analyte solution at lambda max E = molar absorptivity (or molar extinction coefficient) of the analyte, L pmol-1 cm1 I = light path length of the cuvette containing the analyte solution, cm C = concentration of the analyte solution, Mu mol Based on Beer?s law, the absorbance of standard dye solutions at lambda max (A) should be linearly dependent on the corresponding dye concentrations (C). In other words, if a calibration curve (standard curve) is made

Explanation / Answer

Absorbance, A = ?lC

If we plot the graph between A vs C then we get linear line which passing through the orgin and slope of the line is ?l

Where ? = molar extinction coefficient and l = path lenth.

Hence in accordance with Beer's law, the slope of the linear trendline on a plot of absorbance verses concentration is equal to "the product of molar extinction coefficient and path length".

Related Questions

Navigate

• Browse (All)
• Browse B
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.