https://learn-wcsu.ct.edu/bbcswebdav/pid-5419850-dt-content-rid-12625450_1/cours

ID: 820639 • Letter: H

Question

https://learn-wcsu.ct.edu/bbcswebdav/pid-5419850-dt-content-rid-12625450_1/courses/WCSU40649.201440/C%20Subunit%20F1Fo%20ATPase.pdf

please explain the article in simple terms thanks!

1. Provide a brief description of the subunits of the Fo and F1 parts of the ATP synthase and how

they connect.

2. What subunits of the F1Fo ATP synthase at the time of this paper were known to physically rotate?

3. Before this paper was published, other experiments indicated that the c ring also rotates during

ATP synthesis. What was the criticism of those experiments posed by the authors in this paper?

4. What was the basic experimental strategy described in this paper to show rotation of the c

subunit?

5. Describe what is depicted in Figure 2.

6. What type of experiments did the authors perform to indicate that their mutants behave

similarly to wild-type enzyme?

7. Describe what is depicted in A,B, OR C, of Figure 3.

8. Investigate one technique described in the Other Methods section and explain how it works and

provide a reference for your information (web search ok.) (For example

the link require authentication

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