Please help and explain answer. The table below shows the data for purification

Question

Please help and explain answer.

The table below shows the data for purification of a hypothetical protein. Complete the table by filling in the missing values for the specific activity Total Protein Total Activity Specific Activity Yield Purification Step Omg) (units) (units mg level 14700 147000 Homogenization 10.0 100 Salt fractionation 4250 134000 3.15 31.5 91 Number Ion-exchange O 139 1250 109000 8.72 chromatography Number Gel-filtration 50.9 58.1 74800 chromatography 129 Number Affinity 5.10x104 chromatography 1550

Explanation / Answer

you just take the value in the "total activity" column and divide it by the "total protein" column value.

This is because specific activity is in units of "units mg-1" which means units/mg.

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