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Hello Anonymous, Is it possible you can send me a few works cited to go along wi

ID: 82385 • Letter: H

Question

Hello Anonymous, Is it possible you can send me a few works cited to go along with this science project?? Thank You so much.

Summary:

Mangrove Actinomyces strain L3 was screened for the presence of antibacterial activity. Mangrove sediment samples were taken from the sampling sites, Machilipatnam and Nizampatnam. Isolation of Actinomycetes was carried on Starch Casein Agar plates supplemented with rifampicin and cycloheximide. The plates were seeded with a sediment sample and incubated at 28°C for 14 days. After 14 days, colonies were transferred onto SCA slants and incubated at 28°C for 7 days. The isolates were pooled together and cultures which appeared identical were eliminated. Primary and secondary screening of actinomycete isolates were done by cross streak method and well diffusion method respectively. The plates were kept in the refrigerator for about 2h for antibiotic diffusion and then incubated at 37°C. After 24 h, the inhibition zones were recorded and the Isolate L3 was found to have good antibacterial activity against Bacillus subtilis and enterobacter aerogenes.

Explanation of the last table:

The table summarises the values of inhibition zones obtained by different isolates of actinomycetes namely C1, C2, C5, L1, L2, L3, L4, M1, M4 and M6 against gram positive (Bacillus licheniformis, Bacillis subtilis and Staphylococcus aureus) and gram negative (Escherichia coli and Enterobacter aerogens) bacteria. Antibacterial activity against Bacillus licheniformis is maximum with M6 isolate. Isolate L3 was found to have good antibacterial activity against Bacillus subtilis, Staphylococcus aureus and Enterobacter aerogenes. Isolate M4 had good antebacterial activity against escherichia coli.

Explanation / Answer

A Few relevant articles

1. Bizuye, A., Moges, F., & Andualem, B. (2013). Isolation and screening of antibiotic producing actinomycetes from soils in Gondar town, North West Ethiopia. Asian Pacific Journal of Tropical Disease, 3(5), 375–381. http://doi.org/10.1016/S2222-1808(13)60087-0 ( Web link- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027325/)

2. Gebreyohannes, G., Moges, F., Sahile, S., & Raja, N. (2013). Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pacific Journal of Tropical Biomedicine, 3(6), 426–435. http://doi.org/10.1016/S2221-1691(13)60092-1 (Web link- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644569/)

3. Zothanpuia, Passari, A. K., Chandra, P., Leo, V. V., Mishra, V. K., Kumar, B., & Singh, B. P. (2017). Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment. Frontiers in Microbiology, 8, 68. http://doi.org/10.3389/fmicb.2017.00068 (web link- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5263160/)

4. Khandan Dezfully, N., & Gottravalli Ramanayaka, J. (2015). Isolation, Identification and Evaluation of Antimicrobial Activity of Streptomyces flavogriseus, strain ACTK2 from Soil Sample of Kodagu, Karnataka State (India). Jundishapur Journal of Microbiology, 8(2), e15107. http://doi.org/10.5812/jjm.15107 (web link- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376976/)

5. Singh, V., Haque, S., Singh, H., Verma, J., Vibha, K., Singh, R., … Tripathi, C. K. M. (2016). Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications. Frontiers in Microbiology, 7, 1921. http://doi.org/10.3389/fmicb.2016.01921 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138215/)

6. Lingakumar, K., Thangapandian, V., Prabhu, D., Philip Ruban, A. C.. Isolation and characterization of antibiotics producing actinomycetes from soil samples of Senbagadaruvi in Western Ghats. Bioresearch Bulletin. 2011 Feb 2 [last modified: 2013 Oct 16]. Edition 1.(web link- http://bioresonline.org/article/isolation-and-characterization-of-antibiotics-producing-actinomycetes-from-soil-samples-of-senbagadaruvi-in-western-ghats-2/)

7. Kesavan, S. (2015). ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM GARDEN SOIL OF SATHYABAMA UNIVERSITY, CHENNAI.Asian Journal of Pharmaceutical and Clinical Research, 8(6), 110-114. (Web link- https://innovareacademics.in/journals/index.php/ajpcr/article/view/7755/3301)

8. Kumar, P. S., Raj, J. P. P., Duraipandiyan, V., & Ignacimuthu, S. (2012). Antibacterial activity of some actinomycetes from Tamil Nadu, India. Asian Pacific Journal of Tropical Biomedicine, 2(12), 936–943. http://doi.org/10.1016/S2221-1691(13)60003-9 (web link- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621468/)

9. Valan, A. M., Asha, K., Duraipandiyan, V., Ignacimuthu, S., & Agastian, P. (2012). Characterization and phylogenetic analysis of novel polyene type antimicrobial metabolite producing actinomycetes from marine sediments: Bay of Bengal India. Asian Pacific Journal of Tropical Biomedicine, 2(10), 803–810. http://doi.org/10.1016/S2221-1691(12)60233-0 (web link-https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609223/)

10. Kumar, V., Naik, B., Gusain, O., & Bisht, G. S. (2014). An actinomycete isolate from solitary wasp mud nest having strong antibacterial activity and kills the Candida cells due to the shrinkage and the cytosolic loss. Frontiers in Microbiology, 5, 446. http://doi.org/10.3389/fmicb.2014.00446 (web link- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140075/)

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