Design 15 nucleotide long PCR primers from the beginning and end (use the underl

Question

Design 15 nucleotide long PCR primers from the beginning and end (use the underlined sequences) of the sequence (grouped into ten nucleotide blocks), writing the forward and reverse primers in the '5 to 3' direction. 5'- GCTGTCACAG TCGATCGCAT GCACGTCGAG CTACGTAAGT GTGACGATCG ATCGATACGT TTACGATTAC GCGATCAGCT CGCGCATTTA CGATCGATCA GCTTTACGAC AGATTTAT-3' Forward primer: 5'- -3' Reverse primer: 5'- -3' What is the size of the PCR product amplified from the template shown (include units)? Which strand of DNA would result from sequencing with a primer that is top strand sequence? Top or sense strand bottom or non-sense strand both A and B neither A nor B

Explanation / Answer

Ans (27)- i

FORWARD PRIMER -5’GCTGTCACAGTCGAT3’

REVERSE PRIMER -   5’GACGATCGATCGATA3’

Ans-ii

Amplified PCR product should be 57bp long i.e. the size of amplicon is equal to 0.057kb

Ans-28

A- top or sense strand

when we amplify sequence with given primer top strand will amplify. this amplified PCR product is used for sequencing which will give only top strand sequece details.

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