Why is it necessary to chelate the metal ions from solution during the boiling/l

Question

Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100C? What would happen if you did not use a chelating agent such as the InstaGene matrix? What is needed from the cells for PCR? What structures must be broken to release DNA from the cell? Why is it necessary to have a primer on each side of the DNA segment to be amplified? How did Taq DNA polymerase acquire its name? Why are there nucleotides in the master mix? What are the other components of the master mix, and what are their functions? Describe the three main steps of each cycle of PCR amplification and what reactions occur at each temperature.

Explanation / Answer

question 6

Why are there nucleotides in the master mix? What are the other component s of the master mix and what are their functions?

and magnesium which is a cofactor required by DNA polymerase.

=======================================================

question 1

Why is it necessary to chelate the metal ions out of solution during the boiling/lysis step at 100°C? What would happen if you did not put in the InstaGene™ matrix?

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.