Analyze the Forensic DNA data. Answer the following questions and determine the

Question

Analyze the Forensic DNA data. Answer the following questions and determine the identity of the persons inside the shark. Use what you know about patterns of inheritance to make your decisions

Sample Codes:

01=Tissue remains found in shark, 04=Daughter of missing fisherman#1

02= Bone remains found in shark, 05= Son of missing Fisherman #1

03=Tissue remains found in shark, 06= Mother of missing Fisherman# 2

07= Half Brother of missing Fisherman #2

3.a. Sample 06 is from the mother of issing fisherman #2. If sample 01 or 02 are from fisherman #2, what STR variations would you expect to see him inherit from his mother?

b. Do any of the 16 STR loci suggest that sample 06 is from the mother? Do variants match between columns 01, 02 and 06?

c. Do any of the 16 STR loci suggest that sample 06 is not from the mother? Do any of the variants from sample 01 or 02 not match a pattern of inheritance from sample 06.?

4.a Sample 07 is from the half-brother of missing fisherman #2. They share a father. If samples 01 or 02 are from fisherman #2, how many STR variations would you expect the half brothers to share?

b.Do any of the variations at 16 STR loci suggest that sample 07 and samples 01 or 02 have the same father?

c. Do any of the variations at 16 STR loci suggest that sample 07and samples 01 or 02 have a different?

5.Look at your analysis. whose remains are in the shark?

6.what are some data points missing? Do these mssing data points draw any doubts on your conclusion?

Summary of STR Results. Sample 01 06 07 (Tissue) (Bone) (Tissue) (Reference) (Reference) (Reference) (Reference) Locus 14.16 15 16 16 14, 16 15, 17 D3S1358 TH01 6,93 6,93 7, 9.3 29.30 30, 31.2 29, 30 D21S11 29, 30 30, 31 29, 32.2 12,16 12,16 17,19 12, 13 12, 16 D18S51 16, 18 INC 89 17h 13 9, 11. 13.15 Penta E D5s818 8, 10 8, 10 11,12 10, 11 13 12, 13 11,12 13 1213 11 11, 12 D13S317 11, 12 11 9,12 9, 10 10, 12 D7S820 10, 11 10, 11 NR INC 8,9 s, 11 11, 12 D16S539 10, 11 10, 12 CSF1PO 11, 12 INC 2.2,9 Penta D Amelogenin VWA 15, 15, 19 14, 16 16, 19 14, 16 15 15 13,15 14, 15 14, 15 12 D8S1179 (8,9 8,9 TPOX FGA 24,25 22,25 2,24 21,25 24, 25 20, 24 *peak(s) present below reporting threshold; locus not used for comparison peak imbalance present INC locus inconclusive, not used in comparison INR no results Sample 07 represents the fisherman's half-brother; he shares the same father but has a different mother.

Explanation / Answer

3a) Samples 01 and 02 should have at least one match with sample 06 for all of the STR loci. A son inherits half of his variations from his mother.

3b) At least one variation from sample 06 is present at every STR locus of samples 01 and 02.

3c)No. All loci have a pattern of inheritence that is consistent with 06 being the mother.

4a)Each brother will inherit one of the father’s two variations. There is a 50% chance that half-brothers will inherit the same variation at any given locus.

4b)Samples 01, 02 and 07 share a variation in 11 of the 16 loci. This is consistent with the individuals having the same father and a different mother.

5)The observation that all loci between sample 06 and samples 01 and 02 share at least one variation provide the strongest evidence that the remains of Missing Fisherman #2 are in the shark.

6)These are real results from a forensics lab and sometimes DNA reactions do not happen as planned due to degraded samples (such as 03, perhaps), contaminated reactions and other lab errors. Students will recognize that experiments sometimes don’t work. The DNA from the samples in the shark show the most inconclusive data is likely due to the degredation. These inconsistencies do not draw significant doubt on the hypothesis that samples 01 and 02 are from Fisherman #2. Without data for sample 03, however, it is impossible to conclude that this sample is also from Fisherman #2.

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