select all the statements that apply Absorbance measurements in the range of A =
ID: 828206 • Letter: S
Question
select all the statements that apply
Absorbance measurements in the range of A = 0.3-2 are considered the most accurate. Why would absorbance measurements of 0.05 and 2.5 be considered inaccurate? Select all that apply. At A = 2.5, the transmission is too high for an accurate measurement. If the absorbance is too high (A = 2.5), the signal difference between the sample and the reference is too small for an accurate measurement. At A = 2.5, the detector is saturated because too much light is reaching the detector. The difference in the absorbance of the sample and reference is too small at A = 0.05. At A = 2.5, a sufficient amount of light does not reach the detector for an accurate measurement.Explanation / Answer
Absorbance measurement of very dilute and highly concentrated solutions tends to disobey Beer's law, hence not accuarate.
The following statements are true for the same,
At 2.5, a sufficient amount of light does not reach the detector for an accurate measurement.
The difference in the absorbance of the sample and reference is too small at A = 0.05
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