The Ames test is used to compare mutation rates in two treatment groups, one exp
ID: 82827 • Letter: T
Question
The Ames test is used to compare mutation rates in two treatment groups, one exposed to a possible mutagen and one (a control group) that is not exposed. In a standard Ames test, a particular strain of bacteria with a single point mutation (a change in a single nucleotide) is used. Let us say that you want to try a variation on the Ames test to study GTAs and bacteriophages. You develop auxotrophs from a strain of bacteria that produces GTAs. Auxotroph are bacteria that have a mutation that prevents them from synthesizing a necessary nutrient. You then conduct one test exposing the a to GTAs and then a separate test exposing auxotrophs to bacteriophages. Think about how mutations occur and how GTAs and bacteriophages insert their genetic material. What possible results could you find? Drag and drop choices into the bins they describe.Explanation / Answer
THE POSSIBLE RESULT FOR GTA PLATES-Genetic analysis reveals no evidence of viral replication genes.
THE POSSIBLE RESULT FOR BACTERIOPHAGE PLATES-Plaques may be present on the original plates.
THE POSSIBLE RESULT FOR BOTH PLATES- There is an increased growth on experimental plates compared with that on control plates.
UNLIKELY RESULT FOR EITHER PLATES- a)There is an increased growth on control group plates compared with that on experimental plates. b) There is No growth on experimental group plates C) There is no growth on control group plates.
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