Specific proteins on or in a cell can be lagged with fluorochromes that base bee

Question

Specific proteins on or in a cell can be lagged with fluorochromes that base been chemically linked to a. antibodies. b. chlorophyll. c. methane. d. Both (b) and (c) are correct. e. All are correct. The heat filter is used to eliminate nonuseful, heat-generating a. infrared rays. b. ultraviolet rays. c. visible spectrum rays. d. Both (b) and (c) are correct. e. All arc correct. Before reaching the ocular, the light generated by the mercury vapor are lamp travels through the a. barrier filter, heat filter, condenser, slide, objective lens, and then the exciter filter. b. exciter filter, barrier filter, slide, condenser, objective lens, and then the heat filter. c. exciter filter, condenser, heat filter, slide, objective lens, and then the barrier filter. d. heat filter, exciter filter, condenser, barrier filter, slide, and then the objective lens. c. heat filter, exciter filter, condenser, slide, objective lens, and then the barrier filter. The wavelength of fluorescent light rays is a. always longer than the exciting wavelength. b. always shorter than the exciting wavelength. c. about the same length as the exciting wavelength. d. sometimes shorter and at other times longer than the exciting wavelength. The barrier filler in the fluorescence microscope is kept in position to block all light rays from getting through. b. allow fluorescent light rays to pass through. c. screen out exciting light rays. d. Both (b) and (c) are correct.

Explanation / Answer

Fluorochromes are widely used for labeling of proteins and antibodies. Protein specific antibodies that labeled with fluorochrome will help to detect location or co localization experiments. Generally fluorescent dye contain isothiocynate group that interact with amino group of proteins. Malemide group containing fluorochrome also used but that require free –SH (sulfa-hydral group). Correct answer is a. Heat filter prevent infrared wavelengths to protect the fluorescent filter from heat. Correct answer is a. Path of light in fluorescence microscopy is as follows: light pass through Exciter filter and condensed and heat filter remove the infrared wavelength. Than this pass through slide to move objective lens and finally barrier filter. Answer is c. Fluorescence wavelength is always higher than exciting wavelength because exciting wavelength needs more energy to excite the electron of fluorochrome. Shorter wavelength having high energy. For example UV having shorter wavelength than visible. Correct answer is a. Barrier filter remove exciting rays and allow only fluorescence light. Correct answer is d.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.