allege Post Zab Questions me relatively simple answers of extraction bumerA to d

Question

allege Post Zab Questions me relatively simple answers of extraction bumerA to describe how the bumer to the following in order a. List the main components of extraction buffer A and briefly describe the physicalprop. erties of these components. b. Although the cell membrane and nuclear envelope are destroyed during the pulverizing step, how are these membrane molecul es separated from the nucleic acids. What hap- pens to these components after being separated from DNA? 2. There are many proteins embedded in nucleic acids. a. What are these proteins called? b. How are these proteins separated from the DNA? What happens to these proteins after being separated from DNA? Chemistry 116 Grossmont College 14-187 and fat.

Explanation / Answer

Extraction Buffer A is made of following components:

1. NaCl is white color powder, salty, ionic, water soluble, separates DNA from other cellular components

2. NaHCO3 is white crystalline powder, odourless, alkaline with pH 8 approx.

3. Sodium Lauryl Sulphate is amphi-philic. Its sulphate head is hydrophilic and water soluble whereas carbon chain is hydrophobic and doesnt dissolve in water. Odourless white powder.

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