12 48 AM Cell Biology Lab Final Exam Fall 2016 1 A 10-fold daution of the rabbit
ID: 84012 • Letter: 1
Question
12 48 AM Cell Biology Lab Final Exam Fall 2016 1 A 10-fold daution of the rabbit Nesprin antibody, at a starting eonoentration of 10mg would eld which of the following concentrations? a) 01 mg/ml b) 0.01 mg ml 0001 mnghml d) 1 mg/ml 2 use the standard graph below to estimate the molecular weight of paramyosin which migrates 7 centimeters in an agarose gel. 105,000 b) 6.500 d) 55.000 3. Ricin is a highly exic, naturally occurring lectin that is produced in the seeds of the castor oil plant. Ricin th binds complex carbohydrates on the surface of eukaryotic cells. To prevent ricin toxicity you must identify carbohydrate that Ricin binds, To achieve this you perform an experiment on your cheek epithelial cells ike you did cell bio lab using peroxidase linked to ricin. The four carbohydrates you test are glucose mannose and galactose. You find that a purple color develops when you add fucose. glucose and manno in excess but there is no color when you add galactose. Which sugar residue does Ricin normally bind to a) fucose b) glucose c) galactose mannose showed intense 4. In your tissue printing lab some vegetables poorly for conclusion of this observation? staining with the protein stain (red). Which statement below is the best compared There are low quantities of peroxidase to the amount of total protein present b) There are high quantities of peroxidase compared to the amount of total protein present c) There are equal quantities of peroxidase and other present observatio d) You cannot make a conclusion about peroxidase and protein quantities based on this structure in the trachea contains the highest concentration of tubulin? cilia of the epithelium cartilage layer c) muscle layer d) nuclei of the epitheliumExplanation / Answer
9. D. Tbs buffer (reason: tbs buffers in various compositions are used in various Immunochemistry reactions as washing buffers)
10. D. Chloronapthol and tmb (reason: 4chloro 1napthol is used as substrate in analytical purose, TMB is used in elisa and other tests involving horseradish peroxidase)
11. B. (resson: blood cells are suspended in plasma)
12. True
13. False (reason: it is a surfactant and it denaturates proteins)
14. False (reason: as gorilla is mare closely related to human than chicken, gorilla albumin will have stronger response than chicken albumin when treated with human ab)
15. False. (reason : eosin stains cytoplasm not nuclei)
16. False. (reason : stratified sqamous epithelium)
17. True. (reason : By binding to the proteins with high affinity and in high concentrations, the negatively charged detergent provides all proteins with a similar net negative charge and therefore a similar charge to mass ratio.)
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