transferred through this chain of carriers? The Faraday constant, et, is 96.48 k
ID: 84105 • Letter: T
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transferred through this chain of carriers? The Faraday constant, et, is 96.48 kJ/mol. ATP 12. a vertebrate dies, muscles n as they are deprived of ATP, a state called rigor two processes has to do with transport, the other with an ATPase activity) account for molecular basis of the rigor state? Explain in detail 13. Describe the activity of a suicide inhibitor. What can such an inhibitor tell you about the mechanism of an enzyme's action? How could such an inhibitor be used to purify an enzyme which it inhibits? Explain. 14. Normal and sickle globin differ due to a change in of one v to an E. the will of each method for separating these two proteins. I e. if the method work, explain how, and not, explain why not. a. Size exclusion chromatography b. Affinity chromatography c, Iso-electric focusing/2D-PAGE d. SDS-PAGE e. Ion-exchange chromatography 15. Transport of histidine into a bacterial cell was measured at several different histidine concentrations. Sketch a plot of [His] vs. rate of transport. Do the data show that it histidine uptake occurs by simple passive transport by facilitated transport; or is not possible to distinguish? Briefly explain your answer. Histidine (HM) Trans (HM/min) 42.5 240 490 1000 16. Contrary to legend, camels do not store water in their humps, which actually consist of large fat deposits. How can these fat deposits serve as a source ofwater?Explanation / Answer
suicide inhibitor is a type of enzyme inhibitor which binds with an enzyme irreversibly and inactivates it. eg. 5 bromo uracil acts as suicide inhibitor for thymidylate synthase.
we can use these suicide inhibitor for purification. for example if any suicide inhibitor covalently binds with an enzyme and inactivates it. then we can purify this enzyme by affinity purification and after purification we remove the covalently attached inhibitor and refold the protein.
2) because of mutation in one amino acid changes the conformation and size of the protein we can easily seperate these two proteins with size exclusion chromatography.
also their affinity will be different for the matrix used in affinity chromatography. so first sickle cell will be eluted and then normal one.
2D page will seperate it on the basis of size and net charge present on the protein.
SDS page will separate it on the basis of size (mlecular weight).
IEC will seperate it on the basis of their net charge affinity towards ion exchange matrix. less charged molecule will elute first.
3) when concentration of histidine doubles, transport rate will be doubled. its a linear ratio. so by simple passive transport.
4) fat converts into intermediate products of glycolysis G3P and acetyl CoA which ultimately convertsinto CO2 and water and gives energy. this water will be used for further processes.
5) Liver Contains Glucose 6-phosphatase, a Hydrolytic Enzyme Absent from Muscle. these two fates of glycogen in different tissue. in muscle it gives us energy to activate muscle reflexes.
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