1) You are testing some new fluorescent stains to see if they could help tell yo

Question

1) You are testing some new fluorescent stains to see if they could help tell you about how the endomembrane system and the secretory pathway worked. For each of the following stains, state where you think it would be mainly localized in the cell.

a. A stain that bound to G proteins

b. A stain that bound to RNA and DNA polymerases

c. A stain that bound to enzymes that perform primary glycosylation

d. A stain that bound to enzymes that perform secondary glycosylation

e. A stain that binds to hydrolytic enzymes that break down proteins and lipids

f. A stain that binds to clathrin

g. A stain that binds to transfer RNAs

h. A stain that binds to DNA

Explanation / Answer

1. Cell membrane, as G proteins are the transmembrane proteins.

2. Inside the nucleus.

3. Endoplasmic reticulum

4. Golgi bodies

5. Lysosomes

6. Clathrin-coated vesicles

7. Ribosome

8. Nucleus

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