Which of the following statements regarding the polymerase chain reaction is unt

Question

Which of the following statements regarding the polymerase chain reaction is untrue? A. It can increase the amount of DNA in a sample B. It has the potential of diagnosing an infection from a single copy of a gene C. It utilizes DNA polymerases from psychrophilic organisms D. It can amplify DNA of only a few base pairs up to a whole genome E. It essentially mimics DNA replication as it occurs naturally Why are DNA polymerases from thermophilic organisms used in the polymerase chain reaction? A. Because they are required to keep the two strands separated B. Because they cannot add new nucleotides at low temperatures C. Because the primers will not attach to a complementary sequence unless the polymerases warm the reaction tube D. Because they are easier to isolate than psychrophilic DNA polymerases E. Because the priming and extension steps must be carried out at high temperatures to prevent the single strands from reannealing Gel electrophoresis is used to A. Sepa

Explanation / Answer

9. ANS: C. It utilizes DNA polymerases from psychrophilic organisms

Explanation:

Psychrophilic (cold loving) microorganisms, particularly bacteria have a preferential temperature for growth at less than 59° Fahrenheit (15° Celsius).

PCR process which involves cycles of heating to break the hydrogen bonds in DNA and leave single strands that can be copied repeatedly.

10. ANS: E. Because the priming and extension steps must be carried out at high temperatures to prevent the single strands from reannealing.

Explanation:

Biological activity at high temperatures of DNA polymerases found in thermophiles (bacteria that live in hot springs). Thermophile DNA polymerases work at 100C, a temperature at which DNA is denatured (in linear form).

11. ANS: A. Separate DNA Fragment.

Explanation:

Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. Proteins can be separated according to their size and their charge (different proteins have different charges).

12. ANS: B. A negative charge.

Explanation:

DNA is negatively charged because the phosphate group which present in the DNA is negatively charged (PO4, 3- is phosphate group is negatively charge on oxygen).

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