Which of the following media is not used to specifically test of bacteria can ut

Question

Which of the following media is not used to specifically test of bacteria can utilize proteins? a. SIM agar b. Methyl Red Voges (MR - VP) broth c. Gelatin agar d. TSI agar e. broth Which organism will give a negative result to the catalase test? a. b. c. d. A bacteria culture is grown on blood agar and produces a green clearing around the growth. The clearing is a. a none of inhibition b. alpha hemolysis c. beta hemolysis d. gamma hemolysis A strain of Escherichia coli is sensitive to the antibiotic. The culture is heated, cooled, and exposed to a plasmid DNA. A loopful of the culture is spread on a trypticase agar with ampicillin and incubated. Colonies of E coli grow on the plate. What has most likely occurred? a. E coli has mutated allowing it to grow on the medium. b. E. coli has destroyed the ampicillin c. E. coli has been transformed by the plasmid DNA d. E. coli has been transduced by the plasmid DNA. coli is a. an obligate b. a facultative anaerobe c. an obligate anaerobe d. a microaerophile e. All the above are correct Which statement is false about the API 20E System? a. The system can identify more than 120 different enteric bacteria and other gram negative bacteria. b. Is a miniaturized version of 20 different biochemical procedures. c. A number can be calculated from the results that provide an ID number that can determine the species of enteric bacteria. d. It is a system that determines the ability of organisms to utilize 20 different kinds of carbons sources. SIM medium tests for a. Sugar, Inositol and Mannitol use b. Sugar, indole and mannitol use c. H_2S_ indole and motility d. Staphylococcus, indole and Mycobacterium. e. None of the above. Mannitol Salt agar is selective for a. Staphylococcus b. Streptococcus c. Lactobacillus d. Pseudomonas e. All of the above grow on MSA Most bacteria derive their carbon and energy needs from organic molecules and are therefore c. Autotrophs b. Chemolithotrophs c. Chemoorganotrophs d. Photoheterotrophs e. none of the above

Explanation / Answer

21. Ans: D. TSI agar is not used to test if bacteria can utilise protein.

22. Ans. B. Clostridium Perfringens will give negative result to catalase test.

23. Ans. C. The clearing is beta haemolysis.

24. Ans. C. E.Coli has been transformed by plasmid DNA.

25. Ans. B. . E.Coli is a facultative anaerobe.

26. Ans. D. The false statement on API-20E is----

     It is a sysyem that determines the ability of organisms to ulilise 20 different kind of     

      carbon sources.

27. Ans. C. SIM medium tests for H2S, indole, motility.

28. Ans. A. mannitol salt agar is selective for Staphylococcus.

29. Ans. C. Most bacteria derive their carbon and energy needs from organic molecules and are therefore Chemoorganotrophs.

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