List the following events in the pre-RNA processing of a one intron–two exon gen

Question

List the following events in the pre-RNA processing of a one intron–two exon gene in correct sequence order:
• Attachment of snRNP U1 to the 5' splice site
• Transesterification reaction at the branch point adenine
• Transcription of the DNA template into the pre-mRNA molecule
• Recognition and binding the 3' AAUAAA sequence by specific protein factors
• Cleavage at the poly(A) site
• Addition of the 5' cap
• Export to the cytoplasm
• Addition of the poly(A) tail
• Release of lariat structure
• Splicing together of exons

Explanation / Answer

•Transcription of the DNA template into the pre-mRNA molecule

• Addition of the 5' cap

• Addition of the poly(A) tail

• Export to the cytoplasm

• Attachment of snRNP U1 to the 5' splice site

• Transesterification reaction at the branch point adenine

• Recognition and binding the 3' AAUAAA sequence by specific protein factors

• Cleavage at the poly(A) site

• Release of lariat structure

• Splicing together of exons

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