1. What isthe difference between an enzyme \"cofactor\" and an enzyme \"coenzyme

Question

1. What isthe difference between an enzyme "cofactor" and an enzyme "coenzyme"?

2. What are the general properties of enzymes?

3. Define these: Transition state, reaction coordinate, rate determining step.

4. Draw and label a reaction coordinate diagram for an uncatalyzed reaction, S->P, and the same reaction catalyzed by an enzyme E.

5. Fifteen ug of an enzyme of Mr 30,000 working at Vmax catalyzes the conversion of 60 umol of substrate into product 3 min. What is the enzyme's turnover number? Note: Mr is molecular weight.

6. For the reaction (below), the Michaelis-Menten constant, Km, is actually a summery of three terms. What are they? Explain what the terms mean? How is Km determined graphically?   

7. A biochemist obtaines the following set of data for an enzyme that is known to follow Michaelis-Menten kinetics.                                 

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Explanation / Answer

1. Coenzymes are small, organic, non-protein molecules, such as vitamins, that carry chemical groups between enzymes. They are sometimes known as cosubstrates. Coenzymes are substrates for enzymes but are not considered part of an enzyme's structure. Cofactors are non-protein chemical compounds that are bound (either tightly, as in prosthetic groups; or loosely, as in coenzymes) to an enzyme and is required for catalysis. A cofactor can be a coenzyme or a prosthetic group.

2.General Properties

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