Which of the following statements regarding cDNA libraries is false? They are us

Question

Which of the following statements regarding cDNA libraries is false? They are usually double stranded DNA. They are composed of DNA synthesized with the use of reverse transcriptase. They are composed of DNA synthesized from an mRNA template. They contain introns and exons. They contain DNA that is complementary to the mRNA from which it was synthesized. Transgenic animals are those which are Foreign DNA in all cells. Foreign DNA in some of the cells. Foreign RNA in all cells. Foreign RNA in some of the cells. Both A and B The linking of an antibiotic resistance gene within a plasmid vector became possible due to the actions of A 3'-5' exonuclease A 5'-3'exonuclease Restriction enzymes DNA Polymerase DNA Ligase

Explanation / Answer

Answer

19. Option D is the right answer because cDNA library only contains exon part of gene, not intron. An intron is removed during splicing events and cDNA is formed on fully processed mRNA that has only exons.

20. Option E is the right answer because transgenic animal which has foreign DNA in some cells or all of the cells depends on at which stage cells have been introduced into the embryo.

21. Option E is the right answer because ligase is responsible for linking of one DNA fragment to other DNA fragment.

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