A researcher has coated the wells in a multi-well plate with human plasma fibron

Question

A researcher has coated the wells in a multi-well plate with human plasma fibronectin. She introduces integrin-expressing cells into these wells in the presence of different concentrations of a synthetic peptide. She then washes the wells to remove unbound cells and counts the number of cells that remain attached to each well. The results of her experiment are presented in the following schematic graph. The synthetic peptide sequences used in these experiments were GRGDSPC (1) or GGDRSLN (2). Which one of these peptides (1 or 2) would you expect to correspond to curve "a" in the graph? Which curve (a or b) would you expect if a synthetic peptide with the sequence GDGRSPC was used? (C = cysteine, D = aspartic acid, G = glycine, L = leucine, N = asparagine, P = proline, and S = Serine}

Explanation / Answer

intrgrin have RGD sequence. and peptide 1 have the same sequence in it so peptide 1 will bind with coated palte and compete with the cells as the concentration of peptid 1 increases the number of the cells in well decreases (graph b).

so graph a correspond to the peptide 2 and graph b correspond to the peptide 1.

if we use the peptide GDGRSPC, which contain RGD sequence will compete to the cells and showed graph b.

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