1.You do a series of incubations to compare the properties of E. coli DNA polyme

Question

1.You do a series of incubations to compare the properties of E. coli DNA polymerase I
and III. After incubating DNA template prepared from bacteriophage T7 with one or
the other polymerase for 20 min, you add an excess of a second template, phage T3
DNA and continue the reaction for a further 40 min. You compare the amounts of T3
synthesized to the amount of T7. Most of the DNA synthesized in the polymerase I
incubations is found to be T3 DNA, but almost all of the DNA in the polymerase III
incubation is T7 DNA. Explain.

2. Describe a component of normal eukaryotic chromosome replication that requires a
reverse transcriptase-like activity.

Explanation / Answer

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