a) What is the one letter code for the amino acid at the N-terminus of the pepti

Question

a) What is the one letter code for the amino acid at the N-terminus of the peptide?

b) What is the one letter code for the amino acid at the C-terminus of the peptide?

c) Using the one letter code for amino acids, provide the entire intact peptide sequence.

You must determine the sequence of a pentapeptide after running the following experiments: Amino acid composition analysis revealed the following amino acids: 2 Lys, 1 Ala, 1 Trp, and 1 lle. The original peptide underwent one cycle of the Edman degradation and the following PTH-derivative was produced: The original peptide was exposed to trypsin, which gave two free lysine and a tripeptide. Incubation of the original peptide with chymotrypsin gave a free isoleucine and a tetrapeptide. Answer parts a, b, and c below.

Explanation / Answer

(a) one letter code for N-terminal amino acid - K

(b) one letter code for C- terminal amino acid - I

(c) the entire peptide sequence is KKAWI

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