47. You have isolated an octapeptide from a rare fungus that prevents baldness a

Question

47. You have isolated an octapeptide from a rare fungus that prevents baldness and you are interested in its commercial possibilities. Analysis gives the amino acid composition of 2 Lys, Asp, Tyr, Phe, Gly, Ser, and Ala. Cleavage of the peptide with trypsin yields peptides with compositions of Lys, Ala, Ser and Gly, Phe, Lys and a dipeptide. Reaction with chymotrypsin liberates free Asp, a terapeptide with the composition of Lys, Ser, Phe, Ala and a tripeptide that liberates FDNB-Gly on reaction with FDNB followed by acid hydrolysis. What is the sequence?

Explanation / Answer

The sequence should be

Lys-Gly-Phe-Lys-Ser-Ala-Asp-Tyr

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