Which of the following factors affect the resolution (separation) of two compoun

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Which of the following factors affect the resolution (separation) of two compounds: (A) nature of the stationary phase; (B) eluent composition; (C) amount of sorbent used; (D) color of compounds being separated? Which of the following compounds will be the first to elute from a reverse phase silica gel chromatography column? Which will be the last? A mixture of two compounds was spotted on three TLC plates (A-C). The plates were developed using three different eluent systems: Hexane, EtOAc/Hexane 1:20, and EtOAc/Hexane 1:10 (the listed order of eluents does not correspond to the order of TLC plates shown). Which eluent system is the most suitable to perform preparative separation of the two compounds?

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