Which of the following correctly describes the order of events in Polymerase cha

Question

Which of the following correctly describes the order of events in Polymerase chain (PCR): A. Allow primers to bind, heat to denature DNA, cool to allow DNA polymerase DNA. B. Heat to denature DNA, cool to allow primers to bind, heat slightly to allow polymerase to replicate DNA C. Allow DNA polymerase to replicate DNA, cool to allow primers to bind, heat DNA. D. Heat to denature DNA, cool to allow DNA polymerase to replicate DNA, heat allow primers to bind In molecular cloning, the purpose of Polymerase Chain Reaction (PCR) is to: A. Isolate the protein of interest from all other bacterial proteins B. Extract the gene of interest from a source cell C. Amplify the DNA of a specific gene from source DNA D. Put a plasmid into a bacterium Which species of bacteria is frequently used in molecular cloning? A. Y. pestis B. B anthracis C. E. coli D. C trachomatis Plasmids are used in molecular cloning. What is a plasmid? A. A single gene, found cells B. An optional small chromosome, found in human cells C. A single gene, found in bacterial cells D. An optional small chromosome, found in bacterial cells

Explanation / Answer

45). Option B

Polymerase chain reaction involves the three steps:-

46). Option C

Polymerase chain reaction technique used to amplify the particular gene from the DNA sequence. In which forward primer binds at 3’ end and reverse primer binds at the 5’ end of the DNA sequence. This set of primer sequence will define the size of the gene. Multiple copies of this gene will form after amplification.

47). Option C

There are multiple strains of E.coli used for the cloning. It has multiple cloning sites because of that it can be transformed in multiple competent cell (Host) .So this reason makes E.Coli to be used frequently for molecular cloning.

48). Option D

Plasmids are the extra chromosomal DNA in the cytoplasm of the bacteria. It is found circular where the gene of interest added and transformed into the competent cells.

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