In an automated assay that detects and quantifies different viruses present in b
ID: 85429 • Letter: I
Question
In an automated assay that detects and quantifies different viruses present in blood samples, if one particular virus (Virus A) is not able to be detected with the assay (potentially due to a lack of probes..) but a Virus B of the same family that shares many of the same biological characteristics is able to be detected, virus A yields a false positive result. Knowing this, please explain specifically why the false positive result is always of lower intensity than that of a true positive result.
Explanation / Answer
A test is being particularly designed in such a way that the all substrate to be tested reacts or combines with the test material efficiently and strongly with all the test materials which further amplify the effect hence give the more intensity effect..!!
In other case, negative results it is a by chance process where some of the substrate binds or react with the test materials by chance thus giving a very less effect or less signal hence the intensity is less because the binding between the substrate and the test materials is not so stronger and proper to make a good amplification and intensity effect..!!
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