IV. Please solve three of the following problems 1. The molar extinction coeffic

Question

IV. Please solve three of the following problems 1. The molar extinction coefficients for the 8-hydroxyquinolinol complexes of cobalt and nickel are as follows: E at 365 nm 700 nm Co 3529 429 Ni 3228 10.2 What are the molar concentrations of Co and Ni in a solution that has A365=0.846, and A700=0.068, using 1.00 cm cuvet. 2. Thermo Evolution 600 UV-Vis Spectrophotometer (higher model than the one you used in the lab) has the variable spectral bandwidth (effective bandwidth) of 0.2 nm, 0.5 nm, 1.0 nm, 2.0 nm, and 4.0 nm. The grating of the instrument has a linear dispersion is 0.5 mm/nm. For 2.0 nm spectral bandwidth, what the slit width would be required? If you want to characterize the molecule structural changes, choose higher spectral bandwidth, or lower spectral bandwidth? Why?

Explanation / Answer

V)
1)

For Co
A= E*C*l
0.846 = 3529*C*1
C = 2.4*10^-4 (mg/ml)

For Ni
0.846 = 3228*C*1
C = 2.6*10^-4 (mg/ml)

2)

For Co
A= E*C*l
0.068 = 429*C*1
C =1.58*10^-4 (mg/ml)

For Ni
0.068 = 10.2*C*1
C = 6.6*10^-3 (mg/ml)

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