QUESTION 18 Which DNA sequences must a synthetic gene contain in order for the g
ID: 85601 • Letter: Q
Question
QUESTION 18
Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?
DNA ligase sites.
Restriction enzyme sites.
Origin of replication.
Lac operator.
4 points
QUESTION 19
The correct order of DNA segments in an operon is:
Operator, structural genes, promoter.
Promoter, operator, structural genes.
Operator, promoter, structural genes.
Promoter, structural genes, operator.
4 points
QUESTION 20
The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:
---GGATCCATCTGCGAATTC----
---CCTAGGTAGACGCTTAAG---
It contains a BamH1 site (G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end?
EcoR1, followed by EcoR1.
BamH1, followed by BamH1.
BamH1, followed by Eco R1.
EcoR1, followed by BamH1.
4 points
QUESTION 21
When producing a protein from a gene on DNA, the first process to occur must be
mutation
translation
replication
transcription
4 points
QUESTION 22
In an expression plasmid, the origin of replication:
allows the bacteria to duplicate the plasmid.
allows for growth of plasmid containing bacteria in the presence of antibiotics.
is responsible for the replication of mRNA.
Is the position where the base number begins at 1.
4 points
QUESTION 23
Separation of the original DNA strands is due to the action of:
polymerase
helicase
single stranded binding protein
RNA primers
4 points
QUESTION 24
Which of the following terms refers to a region of a chromosome that has a specific function?
Genotype
DNA
Gene
Allele
4 points
QUESTION 25
The tRNA with UAU as the anticodon would be attached to which amino acid?
leucine
tryrosine
isoleucine
serine
A.DNA ligase sites.
B.Restriction enzyme sites.
C.Origin of replication.
D.Lac operator.
Explanation / Answer
Q.18
ANSWER: B. Restriction enzyme sites.
Explanation: During synthesis of DNA, restriction enzyme digestion can generate compatible ends of DNA, capable of being ligated together. The DNA to be cloned can differ from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one plasmid vector to another .
Q.19
ANSWER: B. Promoter, operator, structural genes .
Explanation:
operon comprises of 3 basic DNA components: a.Promoter , enables a gene to be transcribed and recognized by RNA polymerase initiates transcription. b.Operator –it is a segment of DNA , a repressor binds to it and is defined in the lac operon as a segment between the promoter and the genes of the operon.In case of a repressor, the repressor protein physically obstructs the RNA polymerase from transcribing the genes. c.Structural genes which is regulated by operon .
Q.20
ANSWER: C. BamH1, followed by Eco R1.
Explanation: It contains a BamH1 site(G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, so it should be BamH1, followed by Eco R1 .
Q.21
ANSWER: D.transcription
Explanation:Transcription produces mRNA, which is translated into proteins
Q.22
ANSWER: A. allows the bacteria to duplicate the plasmid.
Explanation: An origin of replication to allow the bacterial cells to replicate the plasmid DNA, and a suitable site for cloning
Q.23
ANSWER: B. Helicase
Explanation: Helicase is a protein which attaches to and breaks apart the hydrogen bonds between the bases on the DNA strands. This results in the separation of original DNA strands.
Q.24
ANSWER: C. Gene.
Explanation: A gene is a region of DNA that encodes function. A chromosome consists of a long strand of DNA containing many genes. Specific gene has specific functions.
Q.25
ANSWER: C isoleucine
Explanation: If the tRNA has UAU as the anticodon, the mRNA must have had AUA as the codon. AUA codes for isoleucine.
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