Consider the following list of phosphorylated compounds with their free energies

Question

Consider the following list of phosphorylated compounds with their free energies of phosphate hydrolysis:

Glucose-1-phosphate: -5.0 kcal/mol
PEP: -14.8 kcal/mole
ATP: -7.3 kcal/mol

Which of these molecules could phosphorylate an unknown compound whose free energy of phosphate hydrolysis is -10.3 kcal/mol?

Consider the following list of phosphorylated compounds with their free energies of phosphate hydrolysis:

Glucose-1-phosphate: -5.0 kcal/mol
PEP: -14.8 kcal/mole
ATP: -7.3 kcal/mol

Which of these molecules could phosphorylate an unknown compound whose free energy of phosphate hydrolysis is -10.3 kcal/mol?

Explanation / Answer

PEP only

Since the free energy of phosphate hydrolysis of PEP is larger than that of free energy of phosphate hydrolysis of an unknown compound, the PEP hydrolysis is coupled to phosphorylation of unknown compound.

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