Data analysis 1. Take a picture of your developed gel using the photo documentat
ID: 857317 • Letter: D
Question
Data analysis 1. Take a picture of your developed gel using the photo documentation system and print out a copy of this photo. 2. Confirm the molecular weights of the marker proteins with your instructor. 3. Measure the distance from the top of the gel well to each protein band. (If the band is wide, measure to the center of the band.) Using Excel or another graphing program, create a table containing MW of Markers. Log MW of Markers, Migration Distance. Plot log MW vs. migration distance for the markers and create a linear regression. Next, measure the migration distances of the major bands in your samples and determine their apparent molecular weight by comparison to the standard curve. You should be able to estimate the MW for cytochrome c using this method. logM Whand, = slope x migration distance + y - intercept 4. Provide an average estimated molecular weight for your cytochrome c. How does this compare to the literature value for bovine Cytochrome c?Explanation / Answer
The migration distance can be obtained by measuring the distance from the top of the gel to the band after the procedure is complete. From the table of data, plot a graph for log MW vs migration distance for the known samples. From the equation of the line, the slope and y-intercept can be obtained.
For the unknown sample, determine the migration distance from the experiment and substitute in the formula shown above. Solve for MW to obtain the molecular weight
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