Which of the following BEST describes the composition of the bactierla translati

Question

Which of the following BEST describes the composition of the bactierla translation intitation complex?

2. (Problem 9, part 3) A researcher isolates a mutant variant of the bacterial translation factor EF-G. The mutation allows proper folding of the protein and binding of GTP, but does not allow GTP hydrolysis. At what stage would translation be blocked by this mutant protein? Binding of fMet-tRNA Met to P site. Second aminoacyl-tRNA binding to the ribosomal A site. Formation of peptide bond between amino acids. Movement of ribosome along the mRNA to allow binding of a new EF-Tu-tRNA in A site

Explanation / Answer

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Answer 2: Choice 2 (during translation, the hydrolysis of GTP is required for movement of the amino-acyl tRNA at the A-site of ribosomal assemly as well as translocationof the ribosome towards the 3' end of the mRNA. Thus, failure of GTP hydrolysis will prevent binding of second aminoacyl-tRNA to the ribosomal site A)

Answer 3: Choice 2 (In bacterial translation, the first charged amino-acyl tRNA synthase enters the P-site whereas the A-site remains open for binding of next amino-acyl tRNA synthase. The GTP is indeed bound by IF-2 but it is hydrolyzed at the P-site for movement of mRNA on the ribosome and formation of peptide bond between the two amino acids)

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