studying a novel enzyme X, and you want to characterize this new enzyme. You mea

Question

studying a novel enzyme X, and you want to characterize this new enzyme. You measure the velocity of the reaction with different substrate concentrations and get the following data:

a) Graph the above data. Determine Km and Vmax.

Please be sure to show all excel data tables and graphs used. Please clearly show all steps and equations.

b) Is X an allosteric enzyme? Explain.

c) You decide to do this experiment again, but this time with only one third of the enzyme X concentration used in the first experiment. Estimate Km and Vmax values and explain your reasoning.

Explanation / Answer

a) Graph the above data. From the graph, estimate KM.
Km = (approximately) 10mM

b) Calculate Vmax. Show any equations and calculations.

Using the equation:

Vmax= (approx) 40 mmol/min

c) Is X an allosteric enzyme? Explain.

No, X is not allosteric.
Allosteric enzymes do not follow Michaelis-Menton kinetics.
Allosteric enzymes would give a sigmoidal curve when plotting Vi vs. [S]

d) You decide to do this experiment again, but this time with only one third of the enzyme X concentration used in the first experiment. Draw a new graph on the same graph that you did the first graph on. Estimate Km and Vmax from the new graph.

Km stays the same as in part a. Vmax = 1/3 original Vmax = (approx) 13

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