In PCR, heat is used to denature the DNA strands. Human Insulin is an example of
ID: 86111 • Letter: I
Question
In PCR, heat is used to denature the DNA strands. Human Insulin is an example of a protein produced by recombinant DNA technology. Reproductive cloning is used to introduce foreign genes into a fertilized egg. In vitro Fertilization (IVF) is a technique used in making a reproductive clone. Somatic Cell Nuclear Transfer (SCNT) is a technique used in reproductive and therapeutic cloning. Taq polymerase is used in PCR because it is stable at high temperatures. Bacteriophages, viruses that infect bacteria, can be used as cloning vectors. Eukaryotic genes can be expressed in bacteria using an appropriate promoter. The gene that codes for Green Fluorescent Protein (GFP) is naturally found in aquatic plants. Agarose gel electrophoresis is a technique used to separate DNA fragments by size. Transgenic animals are produced using recombinant DNA technology (AKA: molecular cloning). CRISPR - CAS9 enables precise changes to be made in the DNA of living cells without use of a cloning vector.Explanation / Answer
39. True Denaturation step heat 92C used to denature the strands
40. True -By using rDNA technology Insulin is producing.
41. False - It is cloning of organisms making exact copy of new organisms. eg. Dolly sheep
42. False - Cloning no fertilization done. only transfer of nucleus from somatic cells to ennucleated egg.
43. True
44. True - It withstand upto 98C.
45. True - lambda phage used as cloning vector
46. True - Bacterial promoter required to express the eukaryote DNA in bacteria.
47. False - It present in jelly fish. GFP use in trangenic plant or animals to find our gene of interest is transfered or not.
48. True - Based on size the DNA are seperated.
49. True
50. True
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