A large number of temperature-sensitive replication mutants have been isolated i

Question

A large number of temperature-sensitive replication mutants have been isolated in E. coli. These mutant bacteria are defective in DNA replication at 42 degree C but not at 30 degree C. If the temperature of the medium is raised from 30 degree C to 42 degree C, these mutants stop making DNA in one of two characteristic ways. The "quick-stop" mutants halt DNA synthesis immediately, whereas the "stow-stop" mutants stop DNA synthesis only after many minutes. Predict which of the following proteins, if temperature sensitive, would cause a quick-stop phenotype at 42 C. DNA Polymerase III EcoRI DNA ligese RNA polymerase dNTP

Explanation / Answer

option A is correct.

DNA ploymerase III is the main replicating enzyme in prokaryotes. Morever this is sensitive to temperature.

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