Autologous smooth muscle cells (SMCs) were transfected with plasmid constructs c

Question

Autologous smooth muscle cells (SMCs) were transfected with plasmid constructs containing the full coding sequence for endothelial nitric oxide synthase (NOS3) using liposome mediated gene transfer. The genetically modified SMCs produced high levels of nitric oxide (NO), an agent typically produced by endothelial cells to prevent platelet adhesion. In an effort to improve the blood compatibility of a polymeric vascular prosthesis, genetically modified SMCs were seeded on the prosthesis prior to implantation. Although platelet adhesion was abrogated and patency restored in the short-term, use of the SMC polymeric prosthesis was soon discontinued due to poor performance? What was the likely cause and mechanism of implant failure?

Explanation / Answer

May be the genetically modified SMCs reverted back to their wild type or were transiently modified which caused the platelets to adhere on the polymeric vascular prosthetic causing its blockage resulting in decrease success of implantation.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.