Give two reasons for using a coverslip when prepare a wet mount. What happened t

Question

Give two reasons for using a coverslip when prepare a wet mount. What happened to your field of view as you changed from the 10X objective to the 40X objective? What can you say about the relationship between magnification and field of view? Describe the purposes of compound and dissecting microscopes. Another name for eyepiece is ______. In a compound microscope, the movable part of the microscope on which objectives are mounted is the _____. If you moved the slide to the right and away from you, the image appeared to move _______. Whenever you examine a slide you always begin with the __________ power lens. Assume that your instructor gave you a 15x ocular lens that fit your compound microscope. What would be the total magnification if it were used with the shortest objective? Which objective should be in place when the microscope is put away for the day?

Explanation / Answer

1. The reason for using cover slip when prepare a wet mount are –

2. When change from low power (10x) to high power (40x) on a microscope then the high-power objective lens moves directly over the specimen and the low-power objective lens rotates away from the specimen. As a result, it alters the magnification of a specimen, the light intensity, area of the field of view, depth of field, working distance and resolution.

3. The magnification and the diameter of the field of view have a reverse relationship. If the image gets larger when retina has the same diameter and sees a smaller part of the object at any one time.

Laboratory question

1. A compound microscope is generally used to view small and thin objects like cells.

A dissecting microscope is used to view large specimens such as pollen grains.

2. The another name of eyepiece is Ocular lens and provide a stable platform for eye that it can more easily stay focused on the object and for general microscope it is 10x.

3. Stage. Stage helps to move the slide around by turning two knobs. One moves it left and right and the other moves it up and down.

4. Left .when the microscope slide is moved right then the image will move left and if the slide is moved left then it will move right because microscope reverses everything right to left as well.

5. When examine the slide it always begin by viewing the object through a low power lens (10x) .

6. Magnification of the microscope is the value of multiplying the magnification values of the objective and the eyepiece. 4X is the shortest objective distance.

Lens magnification is , 15*4=60x

7. Scanning objective lens.

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