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Safari File Edit View History Bookmarks Develop Window Help DB 84% Mon Apr 27 5:10 PM Sophie Jo Bangeman a E saplinglearning.com Apple Disney ESPN Yahoo! LiveLoveDIY...ir Makeovers Lobster Bisque Abs USAA Pad Thai Wayland, HI PLyrics Google Maps YouTube Wikipedia News Popular v Mid State U y CHEM 1303 Spl 15 CATE HW Ch diff O 5/6/2015 11:55 PM 92/100 Gradebook Print Calculator Periodic Table 6 of 17 Map The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation Wrepresents adenine or thymine. Enzyme Target sequence Cleavage 5' GAATTC 3' AATTC 3' 3' CTTAAG 5' 3' CTTAA 5' GATATC 3' 5' GAT ATC 3' 3' CTA TAG 5' 3' CTATAG 5' 5' GGCC 3' 5' GG CC 3' 3' CC GG 5' 3' CCGG 5' 5' AAGCTT 3 AGCTT 3 3' TTCGAA 5' 3' TTCGA 5' RGGWCCY 3' GWCCY 3' 3' YCCWGGR 5' 3 YCCWG Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? Note: The complementary DNA substrate strand is omitted clarity. a) 5 ATCGAACTAGGCC b) 5' GATATCCTTCGGCCA 3' EcoR EcoR Hind EcoRV Hind EcoRV Hae O Previous Give Up & View Solution Check Answer Next Ext Hint

Explanation / Answer

(a) 1. EcoRI will cleave it in between G and A. Therefore it should ne 5' ATCG AACTAGGCC 3'

2. Haelll will cause in between GG and CC i,e 5' ATCGAACTAGG CC 3'

3. Hindilll cause in between A and A i,e 5' ATCGA ACTAGGCC 3'

4. EcoRV cause in between 5' ATCGAACT AGGCC 33'

(b) 1. EcoRI will cleave it in between G and A. Therefore it should ne 5' G ATATCCTTCGGCCA 3'

2. Haelllwill cause in between GG and CC i,e 5' GATATCCTTCGG CCA 3'

  3. Hindi cause in between A and A i,e no cleavage

4. EcoRV cause in between T and A i.e 5' GAT ATCCTTCGGCCA 3'

  

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