An in vitro system is used to study replication of a viral DNA genome that is do

Question

An in vitro system is used to study replication of a viral DNA genome that is double-stranded, closed circle. The location of the sites for the restriction MboI on this DNA and the letter names for each MboI fragment are shown in Figure 1. Replication reactions are carried out using viral DNA as a template, extracts of infected coils as a source of enzymes, and dNTPs, all of which are labeled with^32 P. The reaction products are digested with MboI, analyzed by agarose gel. The same reaction is carried out in the presence of increasing concentrations of radioactive 2^, 3^, dideoxyGTP (ddGTP), and the results are shown in lanes 2-4 in Figure 2. The origin of replication of this viral DNA is in which MboI fragment? A A B B C C D D

Explanation / Answer

Viruses generally have one origin of replication. Thus, option F is completely not relevant as it denies the viral DNA having an origin of replication.

The answer for the above answer is C. The origin of replication of this viral DNA is present in the C fragment of MboI . This is because even after subsequent rounds of ddGTP, the C lane is still showing a band unlike the bands at A, B , D and E.

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