Which of the following is not required for E. coil DNA replication? A. gyrase B.

Question

Which of the following is not required for E. coil DNA replication? A. gyrase B. DNA polymerase C. helicase D. DNA ligase E. telomerase Which of the following is not part of an elongating replication fork complex in E. coli? A. Helicase/Primase B. Two molecules of the catalytic subunit of DNA polymerase III C. dnaA protein D Sliding clamp Bidirectional synthesis would be demonstrated by which of the following methods? A. Bacteria were grown on^15 NH_4 until the entire DN contained heavy nitrogen and then transferred the calls to normal, light media for one round of replication. the resulting DNA molecules were separated in density gradients and compared to the expected results for each proposed model. B. Cells were labeled with^3 H-thymidine followed by a pulse of^14 C adenine. This experiment would show radioactive thymidine at both forked junctions and radioactive adenine further away from both junctions. C. Cells infected with bacteriophage were grown in the presence of radioactive nucleotides. When DNA was analyzed in CsCl gradients, the presence of short DNA fragments indicated that discontinuous replication had occurred. D. Cells were labeled with^3 H-thymidine following by a pulse of^H adenine. This experiment would reveal radioactive adenine at both forked junctions and radioactive thymidine further away from the junctions. Which of the following is the first proton to bind to the Ori sequence in the E. coli genome? A Primase B Helicase C. DnaA D. Ligase E. Telomerase

Explanation / Answer

7. Gyrase is required for relaxing positive supercoils

DNA polymerase I is not required for E.coli replication

Helicase is required for Unwinding

DNA ligase is an enzyme that repairs irregularities or breaks in the backbone of double-stranded DNAmolecules

Telomerase is the enzyme responsible for maintenance of the length of telomeres by addition of guanine-rich repetitive sequences

Hence option B is the correct answer

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