When a peptide Q with the amino acid analysis (Ala, Phe, Glyx2, Trp, Lys, Asn, P

Question

When a peptide Q with the amino acid analysis (Ala, Phe, Glyx2, Trp, Lys, Asn, Pro, Arg, Ser, Tyr) is treated with trypsin, three new peptides are formed (amino acid analysis in parentheses): T1(Ala, Phe, Arg, Ser); T2(Gly, Trp, Lys); and T3(Gly, Asn, Pro, Tyr). When peptide Q is treated with chymotrypsin, four peptides are formed: C1(Ala, Asn, Arg, Ser, Tyr); C2(Phe, Lys); C3(Gly, Trp); and C4(Gly, Pro). What can you deduce about the order in which the amino acids in Q are connected? Explain. What are the points of uncertainty?

Explanation / Answer

Trypsin cleaves after Arg/Lys

Chymotrypsin cleaves after Phe/Tyr/Trp

The peptide sequence will be

Phe-Lys-Ala-Phe-Arg-Ser-Tyr-Gly-Trp-Gly-Pro

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.