1. Plasmids are important in biotechnology because they are: engineering A)A veh

Question

1. Plasmids are important in biotechnology because they are: engineering A)A vehicle for the insertion of foreign genes into bacteria for genetic B) Help in bacterial metabolism. C) Surfaces for protein synthesis in eukaryotic recombinants. D) Surfaces for respiratory processes in bacteria. E) Proviruses incorporated into the host DNA. 2. What is the enzymatic function of restriction enzymes? A) to add new nucleotides to the growing strand of DNA B) to join nucleotides during replication C) to join nucleotides during transcription D) to cleave nucleic acids at specific sites E) to repair breaks in sugar-phosphate backbones 3. Integration of 9.8 kb HIV genome into Human Genome could be an example of A) Point Mutation B) Deletion Mutation C) Insertional mutation D) Substitution mutation E) none of the above 4. Bacterial transformation by recombinant plasmids is often identified by which process? A) examining the cells with an electron microscope B) using radioactive tracers to locate the plasmids C) exposing the bacteria to an antibiotic that kills cells lacking the plasmid D) removing the DNA of all cells in a culture to see which cells have plasmids E) producing antibodies specific for each bacterium containing a recombinant plasmid 5. How does a genomic DNA differ from a cDNA? A) A genomic DNA contains both noncoding sequences and coding sequences, whereas a cDNA contains only protein coding sequences. B) The genomic DNA is identical regardless of the cell type used to make it, whereas the content of a cDNAI depends on the cell type used in its construction. c) A genomic DNA contains only noncoding sequences, whereas a cDNA library contains only coding protein sequences. D) Only A and B are correct. E) A, B and Care correct.

Explanation / Answer

1) Plasmids are the heritable material that is extrachromosomal or present outside the nuclear DNA. They can replicate independent of the nuclear DNA. Plasmids are generally used in genetics and biotechnology in the process of cloning and transfer of genes between bacteria. Antibiotic resistance in bacteria is developed mainly because of plasmids.

Plasmids are commonly used as cloning vectors. It is used as a vehicle for insertion of foreign genes into bacteria for genetic engineering. They also help in protein production by harboring the gene of interest. Protein synthesis in eukaryotes takes place in ribosomes and the site for respiratory processes is mitochondria.

So, the answer for this question is option (A).

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