Question 7 1 pts The modularity of transcription factors is taken advantage of i

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Question 7 1 pts The modularity of transcription factors is taken advantage of in a yeast 2 hybrid experiment. Which of the following best supports this statement? O Transcription factors in yeast can function in multiple organisms O Transcription factors really only require a DNA binding doma n to function properly, not the activation/repression domain. O Transcription factors can function as two separate pieces, as long as those pieces are brought in close proximity. O Transcription factors are transcribed as multiple small pieces of RNA that are pieced together prior to translation O The combination of any DNA binding domain and any activation/repression domain from two separate transcription factors produces a hybrid protein that can function like either of the original transcription factors.

Explanation / Answer

ANSWER:

(7). Yeast two-hybrid system is a molecular biology technique used to discover protein-protein interactions and protein-DNA interactions. Most of the eukaryotic transcription factors are modular in a yeast two- hybrid system. It is a key to this system.

Eukaryotic transcriptional factor consists of two domains namely activating domain and the binding domain. Activating domain is the part of the protein that recruits the transcriptional machinery and binding domain is the section of the protein that directly binds DNA.

The modularity of transcription factors is taken advantage of in a yeast two-hybrid system experiment. This is done by tagging two proteins of interest with different domains of a fragmented transcription factor. Then these proteins of interest are in close proximity to one another their will bring their tagged DNA- binding and activation domains close enough to form a functional transcription unit. Then, the functional unit bind to a promoter element upstream of the reporter genes to activate the transcription.

Hence, the correct answer is Transcription factors can function as two separate pieces, as long as those pieces are brought in close proximity. This statement best support the modularity of transcription factors is taken advantage of in a yeast two-hybrid experiment.

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