A complete Homo sapiens gene X has been placed in Escherichia coli cell. Gene X,

Question

A complete Homo sapiens gene X has been placed in Escherichia coli cell. Gene X, is a nuclear gene, but unlike most other H. sapiens genes, does not contain any introns. The most likely reason for Bobs failure was? E. coli ribosomes can not translate mRNA produced by a human gene. The H. sapiens promoter for X is not recognized by sigma factor. Since X is a mitochondrial protein it can not be manufactured in E. coli since it lacks organelles. The genetic code of Homo sapiens is different than that of E. coli. Eukaryotic genes can only be transcribed inside a nucleus. What are the eight different mRNA sequences that could code for the following peptide? Met-Tyr-Met-Tr p-A a-Tr p

Explanation / Answer

Ans 24) in my opinion the homo sapiens x promotor is not recognised by its sigma factor because it does not contain introns which ard Actually region to be transcriped.

Ans 25) AUGUAUAUGUGGGCUUGG

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