a) You generate single and double mutants of the fictional technicolores bacteri

Question

a) You generate single and double mutants of the fictional technicolores bacteria and observe the following phenotypes. Assume that all of the colors occur at different points along a single biochemical pathway.

wt m1 m2 m3 m2,m4 m2,m5
green blue yellow blue red blue

There is not enough information given to reconstruct the biochemical pathway fully. Write down a mutant or double mutant that would allow you to deduce the entire pathway if you knew its phenotype.

b) Technicolores contain a restriction enzyme named TechE1 that defends the bacteria from viral infection by bacteriophage. However, the similar vividcolores bacteria has no restriction enzyme and is easily infected. Technicolores' own DNA does not get attacked by TechE1 because of methylation.

i) Virus is taken from infected vividcolores. can this virus infect technicolores? yes or no

ii) some technicolores manages to get infected somehow. can this virus taken from these cells infect vividcolores? yes or no

iii) can the above virus (from technicolores) infect other technicolores? yes or no

iv) explain your answer to the last question

Explanation / Answer

I can answer part b for you b) i) No, technicolores' restriction enzyme, TechE1 will destroy the viral DNA ii) Yes, vividcolored never had a restriction enzyme and can be infected iii) Yes. The virus likely survived because the cells enzymes "accidentally" methylated the viral DNA before they could be destroyed. These viruses have immunity to TechE1.

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