(2 Points) Certain drugs are commonly used to study mitochondrial respiration. T
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(2 Points) Certain drugs are commonly used to study mitochondrial respiration. These drugs include rotenone, oligomycin, and the mitochondrial uncoupler dinitrophenol. Rotenone inhibits the transfer of electrons from Complex Ito ubiquinone, oligomycin inhibits Complex V/ATP Synthase by preventing the re-entry of protons into the matrix, and dinitrophenol leads to the uncoupling of oxidation from phosphorylation of ADP (meaning that less ATP is produced). If you were interested in studying the ATP production through glycolysis only, which of these inhibitors would be most useful? Why?Explanation / Answer
The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling.
Dinitrophenol is mainly used as a chemical intermediate in the production of wood preservatives and sulfur dyes.
So retenone is most useful inhibitor in the ATP production as in can inhibits the transfer of electrons from Complex I to ubiquinone which is initial process in ATP production.
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