Three different workers were responsible for preparing the coleslaw during busin

Question

Three different workers were responsible for preparing the coleslaw during business hours. Jill prepared the coleslaw in the morning. Steve prepared the coleslaw in the afternoon and Jack made the coleslaw in the evening. Using the information below, A) Calculate the Staph CFU/gram of coleslaw for each sample, B) determine who might have contaminated the coleslaw. 1 gram of coleslaw was used from each sample. Origin of contamination: A bioengineer is running fermentation experiments to produce a new biofuel. The biofuel is produced as a byproduct of normal metabolism of lactose by a newly identified fecal coliform bacterial species. Currently the yield of biofuel is half the expected amount. The engineer suspects that there may be another bacterial species contaminating the recent fermentation. Use the information below to answer the following: Design an experiment using only what we have learned in class to determine A) If there is another bacterial species contaminating the fermentation and B) how to determine if the contaminating bacterium is affecting the biofuel yield.

Explanation / Answer

2) A) The Staph CFU per gram of coleslaw can be calculated by multiplying the number of colonies with the dilution plated on MSA agar.

Therefore, the Staph CFU per gram of coleslaw for the samples are,

Morning sample = 1 x 10 1 = 10

Afternoon sample = 230 x 10 5 = 2.3 x 10 7

Evening sample = 45 x 10 4 = 4.5 x 10 5

B) Staph CFU is more in the afternoon sample. Therefore Steve might have contaminated the coleslaw.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.