Dialysis and chromatography both can be used to separate different molecular spe

Question

Dialysis and chromatography both can be used to separate different molecular species. Which of the following features is NOT an advantage of chromatography compared to dialysis? A1. Chromatography can distinguish between very large molecules, whereas dialysis cannot. A2. Chromatography can separate a sample into three or more fractions each with a different protein of interest, whereas dialysis separates a sample into only two fractions. A3. Chromatography can be used to purify precipitated proteins, whereas dialysis cannot. A4. Chromatography can be used to separate molecules on the basis of charge, whereas dialysis cannot. A5. All of the above are valid advantages. At pH 7, Protein P has a positive charge and Protein N has a negative charge. Which of the following statements is TRUE regarding how these proteins bind to a column in ion-exchange chromatography? A1. Binding of either protein to the column will not change much if we change the pH of the solution. A2. Either cation-exchange or anion-exchange chromatography could be successfully used to separate Protein P from Protein N. A3. A high concentration of free salt in the solution will make both of these proteins bind more tightly to the column. A4. Protein P will bind more tightly than Protein N to an anion-exchange column. A5. All of the above are false.

Explanation / Answer

Question M4-HW-03

Answer is

A5. All of the above are valid advantages.

All the listed points are valid advantages of chromatography compared to dialysis.
Question M4-HW-04

Answer is

Either cation exchange or anion exchange chromatography could be successfully used to separate Protein P from Protein N.

As protein P is positively charged, it can be purified with the help of Cation Exchanger, protein N will not bind to this exchanger, so the protein will be eluted out.

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