This Table shows assay data from a purification procedure. Which statement is mo

Question

This Table shows assay data from a purification procedure. Which statement is most likely TRUE? A1. The gel - filtration step increased the specific activity but not the yield. A2. Of the three chromatography steps, the affinity column step had the biggest drop in yield. A3. After the CM - cellulose step, the purification factor reached over 1000. indicating effective purification. A4. Most of the protein removed in the gel - filtration step was likely enzyme that had unfolded during the CM - cellulose step. A5. All of the above are false. Based on the purification table from Question M4 - HW - 07, which statement below reports a value that is CORRECTLY calculated? A1. The purification factor after the affinity column step was 350. A2. The specific activity after the centrifugation step was 20 units. A3. The yield after the CM - cellulose step was 82.5%. A4. The yield after the gel filtration step was 1%. A5. All of the above statements report incorrect values.

Explanation / Answer

M4-HW-07

Lets look at each statements.

A1: This cannot be true, because the gel filtration step did not increase specific activity.

A2: This statement is true.

A3: Purification factor = ( Initial amount of protein / Final amount of protein)
For the Cm cellulose step, purification factor = (20000/600) = 33.33
Therefore, this statement is not true.

A4: This statement is not true, since affinity column is protein specific.

A5: This statement is incorrect, since A2 is correct.

Therefore, the correct option is A2.

Purification Stage Yield reduction (%) Specific activity Supernatant from centrifugation 0 20 Affinity Column fraction 95.00 350 CM-cellulose fraction 40.00 550 Gel filtration fraction 66.67 550

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