Which of the following statements is TRUE regarding specific activity during pur

Question

Which of the following statements is TRUE regarding specific activity during purification? If specific activity remains the same for each purification step, this indicates the enzyme is not being destroyed by the purification procedure. Specific activity has a value of 100% for a completely pure enzyme. In typical purifications, specific activity will almost always decrease if you increase the number of purification steps. "Specific activity" differs from "activity" because specific activity refers only to that specific enzyme being discussed. All of the above are false.

Explanation / Answer

Dear student.

Statement A4 is true.

Specific activity is the ratio of activity of an enzyme per milligram of total protein (U/mg). It provides a measurement of enzyme purity in the mixture. So statement A2 is false.

A1 is false, even if specific activity remains same, it does not mean enzyme sis not being destroyed during purification step. Enzyme even bears non protein part which may get washed off during purification.

A3 is false, Total activity decreases during purification steps and specific activity increases or remain constant during purification steps. During purification unwanted proteins gets washed away, and the activity of desired protein remains enriched. As a result of which ratio of total activity to total amount of protein increases. Specific activity increases with the number of purification steps.

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