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You have a protein that binds Nitrogen gas as a regulatory molecule. However, do

ID: 889844 • Letter: Y

Question

You have a protein that binds Nitrogen gas as a regulatory molecule. However, don't know the nature of the interaction between the protein and the nitrogen molecules. You perform an experiment where you monitor the fractional saturation of the protein at different pressures of nitrogen gas, measured in atmospheres. Here is your data:

N2

What is the Hill coefficient for nitrogen gas?

A.) 1.2
B.) 1
C.) 0.75
D.) 0.8
E.) 0.90
F.) 2

N2

Pressure Gauage Reading 1/2 1 ATM 2/3 2 ATM 4/5 4 ATM 8/9 8 ATM 10/11 10 ATM

Explanation / Answer

Option E: 0.90 is the Hill coefficient for nitrogen gas. At 10 ATM pressure protiens attains saturated point with nitrogen.

Hill coefficient describes the fraction of the macromolecule saturated by ligand as a function of the ligand concentration. It is used in determining the degree of cooperativeness of the ligand binding to the enzyme or receptor.

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